4V3R
Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Spacegroup name | I 4 |
| Unit cell lengths | 129.910, 129.910, 70.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.860 - 1.950 |
| R-factor | 0.17576 |
| Rwork | 0.173 |
| R-free | 0.22917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4db6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.880 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 41831 | |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 98.1 | 98.2 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM CHLORIDE 0.1 M TRISHCL, PH 8.5 30 % W/V PEG 4000 |
