4V3E
The CIDRa domain from IT4var07 PfEMP1 bound to endothelial protein C receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.019, 56.019, 250.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.500 - 2.900 |
| R-factor | 0.2487 |
| Rwork | 0.247 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.900 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.880 |
| Number of reflections | 10832 | |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 8.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M NANO3, 0.1 M BTP PH 7.5, 20% PEG 3350 |
