4V35
The Structure of A-PGS from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-23 |
Detector | MARRESEARCH |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 94.180, 94.180, 166.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.695 - 2.300 |
R-factor | 0.1758 |
Rwork | 0.175 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.160 | 1.340 |
Number of reflections | 33909 | |
<I/σ(I)> | 12.34 | 2.01 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 7.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.4 | 0.085 M NAAC PH 6.4, 1.6 M (NH4)2SO4, 20% (V/V) GLYCEROL |