4V31
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Deoxyuridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.809, 59.993, 88.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.410 - 1.800 |
| R-factor | 0.16656 |
| Rwork | 0.164 |
| R-free | 0.21855 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.868 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 28709 | |
| <I/σ(I)> | 8.71 | 2.33 |
| Completeness [%] | 98.8 | 97.4 |
| Redundancy | 2.87 | 2.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CITRIC ACID PH 3.5, 25 %(W/V) PEG 3350 |
