4V2U
Apo-structure of alpha2,3-sialyltransferase from Pasteurella dagmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-29 |
| Detector | PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.724, 56.590, 79.542 |
| Unit cell angles | 90.00, 111.95, 90.00 |
Refinement procedure
| Resolution | 45.110 - 2.710 |
| R-factor | 0.19018 |
| Rwork | 0.186 |
| R-free | 0.26099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ilv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.646 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.870 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.110 | 0.630 |
| Number of reflections | 12618 | |
| <I/σ(I)> | 10.4 | 1.9 |
| Completeness [%] | 98.8 | 97.3 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.3 MG/ML OF PROTEIN INDEX SCREEN CONDITION D9 (0.1M TRIS PH 8.5, 25% W/V POLYETHYLENE GLYCOL 3350) |
