4V1L
High resolution structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-31 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 102.250, 102.525, 109.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.830 - 1.750 |
R-factor | 0.15956 |
Rwork | 0.158 |
R-free | 0.17975 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v1k |
RMSD bond length | 0.011 |
RMSD bond angle | 1.455 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.190 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.130 | 1.380 |
Number of reflections | 58166 | |
<I/σ(I)> | 15.8 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1 M SODIUM CITRATE, 0.1 M MES PH 6.5, 30% GLYCEROL WAS ADDED IN ABOVE CONDITOIN FOR TEH CRYOPROTECTANT |