4V17
Structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 131.093, 131.093, 105.199 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.160 - 2.000 |
| R-factor | 0.20403 |
| Rwork | 0.203 |
| R-free | 0.22875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET DERIVATIVE FROM 2.28 A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.170 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 1.500 |
| Number of reflections | 26385 | |
| <I/σ(I)> | 12.5 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.9 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.2 M NA2 CITRATE, 0.1 M HEPES PH 7.5 CRYO 30% GLYCEROL IN ABOVE CONDITION |
