4V14
Structure and function analysis of MutT from the psychrofile fish pathogen Aliivibrio salmonicida and the mesophile Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Collection date | 2008-03-03 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.150, 81.150, 90.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.001 - 2.420 |
| R-factor | 0.2302 |
| Rwork | 0.226 |
| R-free | 0.30510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a6v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.708 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 250.000 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 11909 | |
| <I/σ(I)> | 21.1 | 2.8 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M LITHIUM SULPHATE, 0.1 M TRIS PH 8.5, 1.26 M AMMONIUM SULPHATE |
