4V0H
Human metallo beta lactamase domain containing protein 1 (hMBLAC1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.950, 67.130, 67.900 |
| Unit cell angles | 109.31, 105.40, 90.17 |
Refinement procedure
| Resolution | 45.958 - 1.790 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.960 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.120 | 0.810 |
| Number of reflections | 90649 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 95.5 | 92.6 |
| Redundancy | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS PH5.5 0.18 M AMMONIUM ACETATE 25% PEG 3350 |
