4V0G
JAK3 in complex with a covalent EGFR inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.140, 99.032, 111.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.920 - 3.000 |
| R-factor | 0.25359 |
| Rwork | 0.252 |
| R-free | 0.29465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.989 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.920 | 3.240 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 13200 | |
| <I/σ(I)> | 4.8 | 1.7 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
