4UZV

Structure of a triple mutant of ASV-TfTrHb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Spacegroup name	P 41 2 2
Unit cell lengths	78.367, 78.367, 89.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.400
R-factor	0.30202
Rwork	0.300
R-free	0.34904
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bmm
RMSD bond length	0.014
RMSD bond angle	1.175
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.130	0.320
Number of reflections	4156
<I/σ(I)>	25	5.8
Completeness [%]	99.8	99.7
Redundancy	14.2	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1