4UZL

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM I MYRISTOLEATE COMPLEX - 2.1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-04
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 2
Unit cell lengths	74.123, 170.723, 61.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.360 - 2.100
R-factor	0.21453
Rwork	0.214
R-free	0.25332
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.358
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	74.000
High resolution limit [Å]	2.100
Rmerge	0.070
Number of reflections	46049
<I/σ(I)>	16.7
Completeness [%]	99.2
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		10 %W/V POLYETHYLENE GLYCOL 6000, 0.1 M CITRATE PH 5.0