4UZC
KSHV LANA (ORF73) C-terminal domain, spiral: hexagonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 103.180, 103.180, 228.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 89.360 - 3.700 |
R-factor | 0.20278 |
Rwork | 0.201 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ypy |
RMSD bond length | 0.001 |
RMSD bond angle | 0.508 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (PHASER-MR) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.360 | 3.840 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.230 | |
Number of reflections | 7874 | |
<I/σ(I)> | 18.53 | 1.92 |
Completeness [%] | 95.9 | 99.1 |
Redundancy | 29.7 | 31.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.4 UL OF 31.5 MG/ML PROTEIN IN 5 MM BISTRIS-CL, 5 MM DTT, PH 6.5 WERE AS SUCH EQUILIBRATED AGAINST A RESERVOIR SOLUTION OF 170 MM LITHIUM ACETATE AND 18% (W/V) PEG3350 IN A SITTING DROP SETUP AT 20 DEGREE CENTIGRADE. AFTER TWO DAYS, CRYSTALS WERE DETACHED FROM THE CARRIER PLASTIC BY ADDING 1 UL OF 2 M AMMONIUM FORMATE. |