4UZ7
STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM VI - 2.2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-15 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 62.863, 79.925, 160.004 |
| Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
| Resolution | 79.800 - 2.200 |
| R-factor | 0.18397 |
| Rwork | 0.183 |
| R-free | 0.22864 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.453 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.120 |
| Number of reflections | 40126 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 99.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20 %V/V GLYCEROL 24 %W/V PEG4000 0.160 M MGCL2 0.080 M TRIS-HCL PH 8.5 20MM SOS |
