4UZ6

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM V - SOS COMPLEX - 1.9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	66.455, 69.953, 195.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.900 - 1.900
R-factor	0.19518
Rwork	0.195
R-free	0.23513
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.744
Data reduction software	iMOSFLM
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.400
High resolution limit [Å]	1.900
Rmerge	0.090
Number of reflections	72075
<I/σ(I)>	7.7
Completeness [%]	99.2
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20 %W/V PEG3350, 0.2 M AMMONIUM SULFATE 20MM SOS, pH 7.5