4UZ3
Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 105.490, 105.490, 105.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.589 - 1.750 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.010 | 0.870 |
| Number of reflections | 39631 | |
| <I/σ(I)> | 20.3 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.3 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH6.5, 1.6 M AMMONIUM SULFATE, 5% DIOXANE |
