4UZ2
Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Collection date | 2013-11-27 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 122.930, 122.930, 76.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.796 - 2.500 |
R-factor | 0.2163 |
Rwork | 0.212 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.549 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.810 |
Number of reflections | 20666 | |
<I/σ(I)> | 24.14 | 3.2 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 11.7 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 28& PEG MME 2000, 0.1M POTASSIUM THIOCYANATE |