4UY6
Crystal structure of Histidine and SAH bound Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2014-07-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.045, 71.045, 124.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.612 - 2.040 |
| R-factor | 0.1853 |
| Rwork | 0.180 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uy5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.040 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 21081 | |
| <I/σ(I)> | 60 | 8.6 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 14.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M TRIS PH9.0, 20% PEG 8K, 0.05M MGCL2 |
