4UY5

Crystal structure of Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	295
Detector technology	CCD
Collection date	2012-08-18
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 21 2
Unit cell lengths	74.641, 74.641, 139.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.127 - 1.997
R-factor	0.1987
Rwork	0.195
R-free	0.24070
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.053
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.120	0.340
Number of reflections	28410
<I/σ(I)>	39	3.7
Completeness [%]	99.4	98.4
Redundancy	13.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		0.1 M IMIDAZOLE PH9.0, 4.2 M AMMONIUM ACETATE