4UX3
cohesin Smc3-HD:Scc1-N complex from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.151, 94.801, 284.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.27663 |
| Rwork | 0.274 |
| R-free | 0.32861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xex |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.778 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.110 | 1.500 |
| Number of reflections | 15350 | |
| <I/σ(I)> | 17.7 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.6 | 26.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
