4UW5
Human galectin-7 in complex with a galactose based dendron D2-2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.440, 115.650, 116.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.687 - 2.040 |
| R-factor | 0.2397 |
| Rwork | 0.238 |
| R-free | 0.31850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.412 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.690 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 54509 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350 |
