4UW2
Crystal structure of Csm1 in T.onnurineus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-07-10 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.894, 158.292, 299.275 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 30.657 - 2.632 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.26090 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.608 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.070 | 0.070 |
Number of reflections | 78224 | |
<I/σ(I)> | 40.84 | 4.67 |
Completeness [%] | 98.2 | 98.5 |
Redundancy | 6.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.2 M AMMONIUM CITRATE PH 7.0, 20% PEG3350 |