4UV4
Crystal structure of anti-FPR Fpro0165 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-11 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 128.350, 128.350, 90.601 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.200 - 3.080 |
| R-factor | 0.2446 |
| Rwork | 0.243 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.850 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 4512 | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 10.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL PH 7.5-8.0, 18% PEG8000, 0.18-0.2 M MGCL2 |
