4UV3
Structure of the curli transport lipoprotein CsgG in its membrane- bound conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-31 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.934, 372.847, 161.972 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.590 |
R-factor | 0.29921 |
Rwork | 0.297 |
R-free | 0.34903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uv2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.364 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.680 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.160 | 0.910 |
Number of reflections | 102130 | |
<I/σ(I)> | 6.8 | 1.89 |
Completeness [%] | 91.6 | 27.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 MM TRIS-HCL PH 8.0, 8% PEG 4000, 100 MM NACL AND 500 MM MGCL2 |