4UUX
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.390, 94.030, 132.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.930 - 1.990 |
| R-factor | 0.17741 |
| Rwork | 0.176 |
| R-free | 0.21254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ct8 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.972 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 62924 | |
| <I/σ(I)> | 17 | 2.5 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M NA2SO4, 0.1 M BIS-TRIS PROPANE (PH 6.5) AND 20% (WT/VOL) PEG 3350 |
