4UU4

Crystal structure of LptH, the LptA homologous periplasmic component of the conserved lipopolysaccharide transport device from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Spacegroup name	P 43 21 2
Unit cell lengths	45.000, 45.000, 146.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.020 - 2.751
R-factor	0.2377
Rwork	0.235
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r19
RMSD bond length	0.005
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.140	0.560
Number of reflections	4383
<I/σ(I)>	13.2	4
Completeness [%]	99.8	100
Redundancy	7.8	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20% 1,4-BUTANEDIOL, 0.1 M HEPES (PH 7.5), 0.2 M NACL.