4UTU
Structural and biochemical characterization of the N- acetylmannosamine-6-phosphate 2-epimerase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.714, 82.147, 75.195 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.450 |
| R-factor | 0.14062 |
| Rwork | 0.140 |
| R-free | 0.16915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4utt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.607 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.100 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 78204 | |
| <I/σ(I)> | 18 | 4.3 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M PROPIONIC ACID, CACODYLATE, BIS-TRIS PROPANE BUFFER PH 8.0 AND 25% (W/V) PEG 1500 |
