4UTS
Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-07-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.655, 111.140, 117.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.630 - 2.030 |
| R-factor | 0.17368 |
| Rwork | 0.172 |
| R-free | 0.21326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z1o |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.170 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.820 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.150 | 0.440 |
| Number of reflections | 810104 | |
| <I/σ(I)> | 8.4 | 3.9 |
| Completeness [%] | 98.5 | 91 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 22 % (W/V) PEG (POLY[ETHYLENE GLYCOL]) MONOMETHYL ETHER 3350 AND 0.14 M MG(NO3)2, PH 7.5 |
