4USM
WcbL complex with glycerol bound to sugar site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.050, 115.890, 167.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.160 - 1.820 |
| R-factor | 0.19187 |
| Rwork | 0.190 |
| R-free | 0.22872 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.339 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.160 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.080 | 0.800 |
| Number of reflections | 87041 | |
| <I/σ(I)> | 15.4 | 1.9 |
| Completeness [%] | 99.1 | 93.8 |
| Redundancy | 7.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.5 M NACL, 10 MM HEPES PH 7 |
