4USL
The X-ray structure of calcium bound human sorcin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.369, 111.580, 60.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.340 - 1.650 |
| R-factor | 0.19331 |
| Rwork | 0.192 |
| R-free | 0.22135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1juo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.033 | 0.550 |
| Number of reflections | 20590 | |
| <I/σ(I)> | 21.57 | 3 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PEG 3350 25% W/V, LISO4 0.5 M, 0.1 M TRIS, PH=8.5, CACL2 0.005M |
