4USF
Human SLK with SB-440719
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Collection date | 2014-01-18 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.940, 75.300, 189.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.780 - 1.750 |
| R-factor | 0.19812 |
| Rwork | 0.197 |
| R-free | 0.22815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.770 |
| Number of reflections | 72706 | |
| <I/σ(I)> | 10.6 | 1.8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG6000, 10% ETHYLENE GLYCOL, 0.1 M MES PH 6.0, 0.1 M MGCL2 |
