4URU
The crystal structure of H-Ras and SOS in complex with ligands
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 149.282, 149.282, 200.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.560 - 2.830 |
R-factor | 0.1921 |
Rwork | 0.190 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.740 | 2.980 |
High resolution limit [Å] | 2.830 | 2.830 |
Rmerge | 0.070 | 0.480 |
Number of reflections | 27510 | |
<I/σ(I)> | 30.7 | 5.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.2M SODIUM FORMATE, 2% DMSO |