4URQ
Crystal Structure of GGDEF domain (I site mutant) from T.maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.410, 91.980, 87.590 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 38.370 - 2.500 |
| R-factor | 0.2232 |
| Rwork | 0.222 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3icl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.970 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.690 |
| Number of reflections | 43759 | |
| <I/σ(I)> | 15.5 | 3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M NACL, 10% (W/V) PEG 6000 |
