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Crystal Structure of Staph ParE43kDa in complex with KBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2009-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.449, 136.809, 69.191 |
| Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
| Resolution | 49.730 - 2.290 |
| R-factor | 0.2179 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s16 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 41758 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M AMMONIUM SULFATE, 0.1M TRIS PH 8.5 AND 25% PEG 3350 |
