4UR4
Structure of the type III fish antifreeze protein from Zoarces viviparus ZvAFP13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-30 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.260, 39.690, 44.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.540 - 1.450 |
R-factor | 0.13583 |
Rwork | 0.134 |
R-free | 0.17588 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4msi |
RMSD bond length | 0.015 |
RMSD bond angle | 1.864 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 1.490 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.040 | 0.310 |
Number of reflections | 10321 | |
<I/σ(I)> | 24.2 | 4.7 |
Completeness [%] | 98.0 | 92.5 |
Redundancy | 4.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2.5 M (NH4)2SO4, 0.1 M CITRIC ACID, PH 4.5 |