Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.130, 47.160, 344.079 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 49.154 - 3.000 |
| R-factor | 0.2016 |
| Rwork | 0.198 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bhd |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.280 | 1.450 |
| Number of reflections | 80852 | |
| <I/σ(I)> | 6 | 1.2 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 9.1 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | (NH)2SO4 1.8M, NH4F 0.1M, 2MM PLP |
