4UQD
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.25 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.640, 46.740, 53.070 |
| Unit cell angles | 83.37, 73.53, 65.79 |
Refinement procedure
| Resolution | 42.630 - 1.250 |
| R-factor | 0.133 |
| Rwork | 0.133 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.395 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.830 |
| Number of reflections | 96003 | |
| <I/σ(I)> | 15.21 | 1.58 |
| Completeness [%] | 89.8 | 75.8 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25 % (V/V) PEG 3350, 0.2 M CACL2, 0.1 M HEPES 7.5 |
