4UP0
Ternary crystal structure of the Pygo2 PHD finger in complex with the B9L HD1 domain and a H3K4me2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.910, 53.910, 57.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 1.280 |
| R-factor | 0.13946 |
| Rwork | 0.138 |
| R-free | 0.16611 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xb1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.955 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.090 | |
| Number of reflections | 22695 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 11.6 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 M SODIUM CITRATE, 0.1 M TRIS PH 7, 0.2 M NACL |
