4UOA

Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin Met29Ile mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	99.678, 99.678, 397.212
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	132.400 - 2.500
R-factor	0.23913
Rwork	0.238
R-free	0.26508
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uo4
RMSD bond length	0.007
RMSD bond angle	1.260
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.100	0.650
Number of reflections	26865
<I/σ(I)>	9.4	2.5
Completeness [%]	99.9	100
Redundancy	5.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1