4UNV
Covalent dimer of lambda variable domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-03 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.417, 60.212, 79.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.110 - 1.600 |
| R-factor | 0.19612 |
| Rwork | 0.193 |
| R-free | 0.25793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcg |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.962 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 11350 | |
| <I/σ(I)> | 39 | 4.2 |
| Completeness [%] | 98.0 | 86 |
| Redundancy | 7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M CH3COONA PH4.6, 2M (NH4)2SO4 |
