4UNU
MCG - a dimer of lambda variable domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.340, 34.120, 63.180 |
| Unit cell angles | 90.00, 104.94, 90.00 |
Refinement procedure
| Resolution | 39.940 - 0.950 |
| R-factor | 0.10846 |
| Rwork | 0.107 |
| R-free | 0.12996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcg |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.291 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 89567 | |
| <I/σ(I)> | 21 | 1.9 |
| Completeness [%] | 88.0 | 34 |
| Redundancy | 12 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2M NACL, 2M (NH4)2SO4, pH 8 |
