4UNO
Crystal structure of the ETS domain of human ETV5 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 58.022, 58.022, 84.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.515 - 1.950 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bnc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.520 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.900 |
| Number of reflections | 12146 | |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40% PEG 300, 0.2M CALCIUM ACETATE, 0.1M CACODYLATE PH 6.0 |
