4UNM
Structure of Galactose Oxidase homologue from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.360, 126.630, 107.550 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 53.500 - 1.770 |
| R-factor | 0.18971 |
| Rwork | 0.188 |
| R-free | 0.22819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2EIE (MODIFIED WITH CHAINSAW) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.300 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.060 | 0.630 |
| Number of reflections | 130533 | |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1M NA ACETATE PH 4, 20% PEG 20000 |
