4UNF
Crystal structure of Deinococcus radiodurans Endonuclease III-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.380, 38.558, 37.094 |
| Unit cell angles | 90.00, 89.34, 90.00 |
Refinement procedure
| Resolution | 34.192 - 2.150 |
| R-factor | 0.1553 |
| Rwork | 0.153 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.340 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.790 |
| Number of reflections | 14192 | |
| <I/σ(I)> | 12.7 | 2.5 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.0 M SUCCINIC ACID PH7.0, 0.1 M HEPES PH7.0, 1% W/V PEG-MME 2000 |
