4UNB
THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 6 DAYS INCUBATION IN 5MM MN (STATE 5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.128, 70.666, 107.375 |
| Unit cell angles | 90.00, 119.62, 90.00 |
Refinement procedure
| Resolution | 46.564 - 2.550 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vs8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 44171 | |
| <I/σ(I)> | 17.2 | 2.8 |
| Completeness [%] | 96.6 | 94.8 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PH 6 |
