4UN7
THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA BEFORE INCUBATION IN 5MM MN (STATE 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.570, 70.347, 106.601 |
| Unit cell angles | 90.00, 119.89, 90.00 |
Refinement procedure
| Resolution | 46.195 - 2.700 |
| R-factor | 0.1802 |
| Rwork | 0.177 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vs7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 37832 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6 |
