4UN4
Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.780, 66.816, 186.834 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 47.493 - 2.371 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.694 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.100 | 0.810 |
| Number of reflections | 160249 | |
| <I/σ(I)> | 16.5 | 2.2 |
| Completeness [%] | 99.1 | 91.5 |
| Redundancy | 6.8 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-ACETATE, PH 8.5, 0.3 M KSCN, 15% (W/V) PEG 3350 |
