Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.580, 148.780, 92.150 |
| Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
| Resolution | 57.821 - 3.150 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2I53 AND 4BCG |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.579 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.820 | 3.370 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.190 | 0.870 |
| Number of reflections | 22936 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG3350, 10% ETGLY, 0.1M BISTRIS PROPANE PH6.5, 0.2M SODIUM NITRATE |
