4UMV
CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN THE E2P STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.462, 60.972, 141.523 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 46.082 - 3.200 |
| R-factor | 0.2216 |
| Rwork | 0.218 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rfu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.210 | 1.150 |
| Number of reflections | 15457 | |
| <I/σ(I)> | 8.01 | 1.37 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM MGCL2, 200 MM LITHIUM ACETATE, 17 % V/V PEG2000-MME, 10 % V/V GLYCEROL AND 5 MM BME |
