4UMO

Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65
Unit cell lengths	151.760, 151.760, 56.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.675 - 3.000
R-factor	0.215
Rwork	0.213
R-free	0.24660
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.005
RMSD bond angle	0.894
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.060
Number of reflections	15107
<I/σ(I)>	40.8
Completeness [%]	99.8
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	0.3 M POTASSIUM THIOCYANATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH=5.6, 1 MM EGTA AT 4 DEGREES CELSIUS